Differences in definitive endoderm induction approaches using growth factors and small molecules
نویسندگان
چکیده
منابع مشابه
Definitive endoderm differentiation of human-induced pluripotent stem cells using signaling molecules and IDE1 in three-dimensional polymer scaffold.
Human-induced pluripotent stem cells (hiPSCs) are considered to be potentially able to differentiate into all human cell lineages and thus hold promise as an unlimited source for cell replacement therapies in clinical applications. Definitive endoderm (DE) formation is the first and crucial step in the development of visceral organs such as liver, lung, pancreas and so forth. Therefore, efficie...
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Reprogramming can occur by the introduction of key transcription factors (TFs) as well as by epigenetic changes. We demonstrated that histone deacetylase inhibitor (HDACi) Trichostatin A (TSA) combined with a chromatin remodeling medium (CRM) induced expression of a number of definitive endoderm and early and late pancreatic marker genes. When CRM was omitted, endoderm/pancreatic marker genes w...
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The directed differentiation of iPS and ES cells into definitive endoderm (DE) would allow the derivation of otherwise inaccessible progenitors for endodermal tissues. However, a global comparison of the relative equivalency of DE derived from iPS and ES populations has not been performed. Recent reports of molecular differences between iPS and ES cells have raised uncertainty as to whether iPS...
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15 صفحه اولUsing Small Molecules to Control Stem Cell Growth and Differentiation
Small molecules are routinely used to manipulate signaling pathways during the in vitro culture of cells. Signaling pathways that control cell proliferation and differentiation are important targets for small molecules in the culture of stem cells. Targeting pathways such as the canonical Wnt, transforming growth factor-β (TGF-β) and retinoic acid signaling pathways can be useful to enhance and...
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ژورنال
عنوان ژورنال: Journal of Cellular Physiology
سال: 2017
ISSN: 0021-9541
DOI: 10.1002/jcp.26214